PCR/RFLP Assay for Copy Number of Mutant and Wild-Type Alleles
نویسندگان
چکیده
منابع مشابه
PCR/RFLP assay for copy number of mutant and wild-type alleles.
A PCR method for quantitating the copy number of mutant vs. wild-type alleles in DNA from cell lines is described. The assay can be used to detect a point mutation in any gene that creates or destroys a restriction site. The alleles of interest are amplified by nested PCR and labeled in a second round of PCR. The product is digested with a restriction enzyme specific for that site, resolved on ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96216st02